Eva Armengol Rivero. Curs 2014-15

Resumen del proyecto:
Bacteria  have several mechanisms  to  attack  targeted  cells,  such  asaffecting  the membrane, by internalization etc. A way to affect the membrane is through the effect of toxins that form channels on it. These toxins are produced by bacteria and exported to the environment affecting  the  integrity  of  the  membrane  and subsequently destroying the  cell.  A  genus  capable  to  produce  this  type  of  toxins  is Bacillus spp.,  examples  of species with this ability are Bacillus anthracis and Bacillus thuringensis. Species of Bacillus spp. were found on tobacco cigarettes; inducing to think that could be possible to facilitate the entry of harmful substances into the cell. These substances could  act  as  carcinogenic  molecules,  being  relevant their study. To  study  the  protein pore-forming  activity  Black  Lipid  Bilayer was used.  The  single  channel  conductance and  ionic  selectivity  were  assayed,  showing  a  clear  pore-forming  activity. The  single channel conductance showed a different average of conductance depending on the salt solution, andthe ionic selectivity showed that the protein preferred anions than cations. The  results  confirmedthe  pore-forming activity; this  fact  indicates  the  possibility  that these bacteria could form toxins that could affect human cells. The next step is to purify the protein, determine its molecular height and its molecular structure.

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